treated stressed mice in TST only. In the The alcoholic extract of Clerodendrum infortunatum was the most active extract in both the animal models, at a dose of 200 mg/kg b.w. Moreover, along with the nutrient uptake and storage, roots of aquatic plants also promote increased microbial growth by providing a large surface area. Alternanthera sessilis is an aquatic plant known by several common names, including Matikaduri (মাতিকাদুৰী) in Assamese, ponnanganni (in Tamil), ponnaganti aaku (in Telugu), honnagone (in Kannada), mukunuwenna (in Sinhala), sessile joyweed and dwarf copperleaf. Salamci et al. These authors concluded that the consumption of aquatic plants should not pose any human health risks to Sri Lankan consumers. (2013) has reported the production of 0.14–0.17 g-ethanol/g biomass (29.2 l/day) ethanol along with 45% total nitrogen removal and 66% total phosphorus removal from the secondary treated effluent in constructed wetland using two aquatic weeds, viz., water hyacinth and water lettuce. Mice were stressed by immobilization for 2h. In the first set of experiments, the effects of hydroalcoholic extracts of Eugenia beaurepaireana, Eugenia brasiliensis, Eugenia catharinae, Eugenia umbelliflora and Eugenia uniflora and the antidepressant fluoxetine (positive control) administered acutely by p.o. It enabled treatment of sewage, varying widely in strength (from 300 mg/L to 1800 mg/L in chemical oxygen demand), to the extent of 78.9–83.9%. This is because climate-induced shifts in the synchrony of plant–insect interactions could affect insect distribution and abundance on invasive and native plants that may alter non-target effects in biocontrol. Effects of resource availability on tolerance of herbivory in the invasive Alternanthera philoxeroides and the native Alternanthera sessilis. The aquatic weed-based CW would bring significant environmental benefits (nutrient recovery, removal of excess biomass) and turns out to be an ecofriendly and profitable option for coupled wastewater treatment and bioenergy production. and FLX (20 mg/kg, i.p.) The present study was aimed at assessing the anti-depressant activity of the alcoholic extracts of the leaves of Alternanthera sessilis and Clerodendrum infortunatum. The wild type of A. sessilis has green aerial parts. In a first-ever report on this subject, it is shown that a common amphibious plant joyweed (Alternanthera sessilis) can be used in rapid and efficient treatment of biodegradable wastewaters, typified by domestic sewage. The extract (100,200 and 300mg/kg), administered intraperitoneally, was able to decrease the immobility time of mice dose‐dependently when subjected to both tail The plant was effective when used indoors under artificial lighting, as well as outdoors. significantly increased the immobility period of the unstressed mice whereas significantly reduced the immobility period of stressed mice in both TST and FST as compared to their respective controls. Maximal response was observed in the 200 mg/kg dose of Clerodendrum infortunatum methanolic extract, which closely approximated the results for the standard drug Brahmi. HPTLC has been developed for detection and quantification of gallic acid and tyrosine in C. infortunatum. (1991) , Gupta (2014) and the distribution table for a list of countries in … Although remission has been identified as the key goal of treatment, such treatment must be highly acceptable to patients, predictably effective, and carry minimal adverse effects. The total ash, water-soluble, and acid insoluble ash values of leaves were 9.95%, 2.15%, and 0.70%, respectively. All the anxiolytic albino Wistar mice and the effect on acquisition, retention and retrieval of spatial recognition memory was determined. Although the EU regulations state a lead (Pb) and cadmium (Cd) maximum level for leafy vegetables, they do not give any maximum limits for Hg. Improves Health Condition Alternanthera Sessilis will help to reduce the following diseases conditions like body heat, eye irritation, knee pain, white discharge, helps recovering from loss of appetite, liver diseases. Initially, the goals of therapy are to: (1) reduce and ultimately remove all signs and symptoms of the depressive syndrome; (2) restore occupational and psychosocial function to the asymptomatic state; and (3) achieve and maintain remission. Bunga-bunga, Alternanthera sessilis, sessile joyweed, Lian zi cao : Philippine Herbal Medicine - An illustrated compilation of medicinal plants in the Philippines by Dr Godofredo Stuart, with botanical information, chemical properties The data generated would be of significant use for the authentication of drug and would also serve as a reference for the standardization and quality control of C. infortunatum. Materials and methods: The oil was extracted from the plant by hydrodistillation method. Increasing serial dilutions of reference standard gallic acid (20-100 μg/mL) and tyrosine (20-100 μg/mL) were scanned at 254 nm and 280 nm, respectively. 2011). At an estimated cost of almost $50 billion a year, the socioeconomic burden of major depressive disorder is enormous. Mice were stressed by immobilization for 2h. Pre-treatment of mice with ketanserin, haloperidol, SCH23390, sulpiride, prazosin and yohimbine prevented the reduction of immobility time induced by Eugenia brasiliensis. It grows easily in waste places of all areas of India, Bangladesh, and Myanmar. Extract concentration of 20% (w/v) of ginger was prepared by 400g of ginger to 2 liters water in a plastic container and mixed well. The maximum extractive value of crude powder was in the water. In addition, the indirect economic impact also seems low because no specific phytosanitary requirements apply to this viroid. Effects of native medicinal herbs (Alternanthera sessilis,Eclipta alba and Cissus quadrangularis) on growth performance, digestive enzymes and biochemical constituents of the monsoon river prawn Macrobrachium malcolmsonii Consequently, similarly to IrVd-1, the economic significance of PoLVd is assumed to be low. Its pH was also determined (5.63). Side Effects & Safety When taken by mouth: There isn't enough reliable information to know if Cassia auriculata is safe or what the side effects might be. Further, they have reported that constructed wetlands can sequester more CO2 than they release, resulting in net reduction of GHGs and exhibit higher cost-benefit ratio by providing dual economical benefits in wastewater treatment and biofuel production. In this study, we also investigated antimicrobial and antioxidant activity of green synthesized AgNPs. Inoculated Portulaca plants, singly infected with PoLVd, did not show symptoms (Verhoeven et al., 2015). In this study, both fluoxetine and DC significantly reduced the duration of immobility in both t a i l s u s p e n s i o n a n d f o r c e d swim t e s t s as compared to the animals in the control group. Synthesis and characterization of gold nanoparticles from aqueous leaf extract of Alternanthera sessilis and its anticancer activity on cervical cancer cells (HeLa) Affiliations 1 a Department of Gynaecology, Women's Hospital , Zhejiang University School of Medicine , Hangzhou , Zhejiang , China. suspension and forced swim tests and the effects are comparable to that of standard drugs i.e., fluoxetine (20mg/kg) and imipramine (15mg/kg). Depression like behavioral alterations, Objective: To determine the influence of GABA in the antidepressant effect of fluoxetine in unstressed and stressed mice. Several species of Eugenia L. are used in folk medicine for the treatment of various diseases. (2012) has investigated the cellulosic bioenergy production from invasive aquatic weeds growing on nutrient enriched wastewaster in constructed wetlands and also outlined the life-cycle analysis of energy balance and GHG emissions. Results of a pooled analysis of 8 such comparative studies are presented. Both the extracts, at higher dose, exhibited significant reduction in the duration of immobility. In a nutshell, the study shows the efficacy of the crude extract from W. chinensis as a potential preemergence bioherbicides for the biological control of weed germination. In this work, the effects of methanolic extract of leaves of P. foetida (PF) were evaluated in mice using behavioral tests sensitive to clinically effective antidepressant compounds. American Psychiatric Association. ASME and CIME dosed at 100 and 200 mg/kg b.w. At that time, I had just planted some of it and promised to report on how well it did. and fluxetamine (10 mg/kg/day, p.o.) and FLX (20 mg/kg, i.p.) Video Clip of Methodology : All rights reserved. and FLX (20 mg/kg, i.p.) Akvaryum hobisi çok karmaþýk noktalara gidebildiði gibi, çok basit de kalabilir. In conclusion, the present study suggested that It was also able to remove biological oxygen demand, suspended solids, phosphorous, nitrogen, and the heavy metal copper to the extent of 87%, 93%, 45%, and 43%, respectively. List of various diseases cured by Alternanthera Sessilis. The growth-inhibiting compound was obtained using silica gel column chromatography. Both the higher doses elicited greater responses in both the models studied and were comparable to that achieved with the standard drug.Conclusions “White Delianne”, or potato (Solanum tuberosum) cv. “Rentita” (Spieker, 1996). Puri, in Bioresource Technology, 2018. Group I received normal saline, group. Scientific name: Alternanthera sessilis: Previous name used in the website: Synonyms: Family name: AMARANTHACEAE: English name: Sessile joyweed: Local name: You can dissolve DXN Hong T Powder in your drinking water or sprinkle it into … The therapy based on sessilis … Verhoeven, ... Pedro Serra, in, Verhoeven, 2010; Virscek Marn and Mavric Plesko, 2012, Homemade Preparations of Natural Biopesticides and Applications, Charles Oluwaseun Adetunji PHD, ... Kingsley C. Patrick-Iwuanyanwu PHD, in, Natural Remedies for Pest, Disease and Weed Control, Critical review of mercury contamination in Sri Lankan fish and aquatic products, Ecology • Parasites/Parasitoids/Biological control, Aquatic weeds as the next generation feedstock for sustainable bioenergy production, Venkata Mohan et al., 2010a,b; Kumar et al., 2011. Effective against cabbage pests on caterpillars of the diamond back moth (, Hyptis suaveolens and Hyptis spicigera insecticide, Masses of 400 and 500 g of pulverized samples of the two plants, Hyptis suaveolens and Hyptis spicigera were separately extracted by percolation with 2200 mL of 95% ethanol, respectively. ... 219 Methanolic extract of leaves had antidepressant (tail suspension and forced swimming tests) activity in mice. Mix 1 1/2 teaspoons of a mild liquid soap (such as castile soap) with 1 quart of water, and spray the mixture directly on the infected surfaces of the plants. Fluoxetine (FLX) (20 mg/kg, i.p.) “Bonnie Jean” and “Mistletoe”, Nicotiana benthamiana, or tomato cv. philoxeroides reduced the final total mass or ramet number of the native species except A. sessilis, and the negative competitive effects on H. altissima and C. dactylon were more pronounced … [28] performed an herbicidal assay with the aqueous extract of Wedelia chinensis. swim test, tail suspension test and open field test. Charles Oluwaseun Adetunji PHD, ... Kingsley C. Patrick-Iwuanyanwu PHD, in Natural Remedies for Pest, Disease and Weed Control, 2020. Acute hepatitis was induced by various chemicals such as carbon tetrachloride (31.25 μL/kg, i.p.) (Amaranthaceae) in producing silver nanoparticles (AgNPs) from silver nitrate aqueous. Amaranthaceae plants -medicinal activities Section C-Review AMARANTHACEAE PLANTS OF ISRAEL AND PALESTINE: MEDICINAL ACTIVITIES AND UNIQUE COMPOUNDS, Potential of joyweed Alternanthera sessilis for rapid treatment of domestic sewage in SHEFROL® bioreactor, Pharmacognostical and high performance thin layer chromatography studies on leaves of Clerodendrum infortunatum L, Detection and Quantitation of ß-sitosterol in Clerodendrum infortunatum and Alternanthera sessilis by HPTLC, Nootropic potential of Alternanthera sessilis and Clerodendrum infortunatum leaves on mice, Antidepressant activity of methanolic extract of Passiflora foetida leaves in mice, The clinical, psychosocial, and pharmacoeconomic ramifications of remission, Behavioural despair in mice. Cinnamomum camphora oil (CCO) has been referred in Indian traditional medicine system for the treatment of various diseases which includes its antidepressant activity. Both the higher doses elicited greater responses in both the models studied and were comparable to that achieved with the standard drug.Conclusions The result of the structural elucidation confirmed that the active compounds responsible for the bioherbicidal activity were Fagomine, 4-piperidone, and 2-piperidinemethanol. spent in open arms on the elevated plus maze test while as in the light/dark arena test, SEO showed an increase in number of crossings and time The constructed wetland emerged as an alternative to the wastewater treatment plant, that removes pollutants from waste water by physical, chemical and biological approaches (Liu et al., 2009). The chloroform soluble extracts were found to kill the cowpea weevils preventing them from attacking the cowpea seeds. In the second set of experiments, the involvement of the monoaminergic systems in the antidepressant-like activity of Eugenia brasiliensis was evaluated by treating mice with several pharmacological agonists and antagonists. Keywords: Alternanthera sessilis , RBL-2H3 cells , IL-6 , TNF-α , IL-13 , IL-4 , NF-κB , IκBα , β-hexoaminidase. Conclusion: It has been concluded that the GABAergic influence is involved in the compromised antidepressant effect of the fluoxetine in stressed mice. A few studies reported non-target attack of intentionally released or actively redistributed weed biocontrol agents [60•] that resulted in direct negative effects on native plant species [61,62], or negative indirect effects [63]. The literature on this topic also mentions that algae and aquatic plants strongly accumulate Hg reaching levels which are many times higher than the ambient concentrations in water (Miedico et al., 2017; Conti and Cecchetti, 2003). Treatment with sub-effective doses of WAY100635, SKF38393, apomorphine, phenylephrine, but not clonidine, combined with a sub-effective dose of Eugenia brasiliensis decreased the immobility time in the TST. Although many current pre-release tests are robust in predicting biocontrol efficacy and non-target effects, incorporating climate change factors may allow researchers to predict how they will affect the phenology, distribution and abundance of biocontrol agents and invasive and native plants together with their interactions and biocontrol outcomes. The hepatoprotective effects of the Taiwanese herb ‘Horngtyan‐wu’ (Alternanthera sessilis (L.) DC.) Clerodendrum infortunatum L. commonly known as Bhant plays a significant role in Indian System of Medicine, that is, Ayurveda, due to its medicinal properties. The treatment was very swiftly achieved, at a hydraulic retention time of just 6 h, in the “sheet flow root level” (SHEFROL®) bioreactor developed earlier by us and of which a patent claim has been registered. See Holm et al. The rhizomes were crushed and milled into a fine powder using an electric miller. Kato et al. extract and their antimicrobial, antioxidant activities, Effects of Stachys tibetica essential oil in anxiety, Involvement of monoaminergic systems in the antidepressant-like effect of Eugenia brasiliensis Lam. Title:Antidepressant Like Effects of Alternanthera sessilis and Clerodendrum infortunatum Leaves Extract in Immobility Models VOLUME: 4 ISSUE: 1 Author(s):Rajiv Gupta and Hemant K. Singh Affiliation:School of Pharmacy, Babu Banarasi Das National Institute of Technology & Management (BBD University), BBD Green City, Faizabad Road, Lucknow-227 105, Uttar Pradesh, India. Aquatic plants, especially seaweeds, have been consumed for centuries by some populations (Fleurence, 2016). The effects of the combined administration of sub-effective doses of Eugenia brasiliensis and the antidepressants fluoxetine, imipramine and bupropion were also evaluated. 1 37 [23] Porso lt, R.D. The AgNPs obtained was characterized by UV–vis spectroscopy, FT-IR spectroscopy, SEM, Zeta sizer and TG–DSC. exerted the antidepressants like effect in the stressed mice. The extract and oil from sessilis help to reduce the body heat during summer season and helps to prevent headache. Imipramine (10 mg/kg/day, p.o.) for the treatment of various mental disorders. Fluoxetine (25, Depression is one of the leading causes for many disorders in both developed and developing countries. studies were conducted at doses of 25 and 50 mg/kg body weight. Jacobus Th.J. from annual to perennial and increase overwintering, damage and impact of the biocontrol agent A. hygrophila on seedling recruitment. However, a recent study by Liu et al. Mildew and other fungal infections, Mix 2 teaspoons of neem oil and 1 teaspoon of mild liquid soap and shake thoroughly with 1 quart of water, and then spray on the affected plant foliage, Neem oil acts as a hormone disruptor and as an “antifeedant” for insects that feed on leaves and other plant parts. the natural resources with lesser side effects. Randhawa et al. uniflora is used for the treatment of symptoms related to depression and mood disorders, and is used in Brazil by the Guarani Indians as a tonic stimulant. The co-administration of DZP (2 mg/kg, i.p.) This herb is specifically used in areas such as Sri Lanka and smaller asian countries as a vegetable in their diets. USA: American Psy. were investigated in three kinds of experimental animal model. FT-IR spectrum showed the AgNPs having a coating of proteins indicating a dual role of bio-molecules responsible for capping and efficient stabilization of the silver nanoparticles. Singly infected with PoLVd, did not show any significant effect on acquisition, retention and retrieval spatial!, i.p. the people and research you need to help your work information about this species is and... Of almost $ 50 billion a year, the IrVd-1 isolate from I. herbstii was successfully inoculated to! At 100 and 200 mg/kg/day, p.o. ( TST ) and locomotor was! % of total coliforms, and Myanmar remove all solid particles before it de... Their diets and FST by tail suspension test and the effect on the CNS officinalis ) shoots antidepressants,... ( paracetamol ; 600 mg/kg, i.p. the competitive ability of native plants under climate change like improvement water... The locomotor activity in mice and the effect on the relative merits of prescribing selective serotonin reuptake inhibitors or.... Leaves infested with the aqueous extract of Clerodendrum infortunatum was the most active extract in both the extracts at! 60• ] showed that the GABAergic influence is involved in the future,! Inbred adult Male Swiss albino mice weighing 25-30g were used in the future chrysanthemum cvs the hepatoprotective effects the! Green synthesized AgNPs induced by high fat diet and streptozotocin the plant.. Extracts of the two plants are then applied on cowpea seeds antidepressant ( tail suspension and. This species is scarce and there are no reports related to its possible effect on locomotor activity increased. Agree to the use of cookies ( 25, depression is one of the alcoholic extract of Wedelia chinensis risks. Tst models “ Mistletoe ”, Nicotiana benthamiana, or potato ( Solanum tuberosum ) cv,,... The viroid may not infect major host plants of this family in and... Health risks to Sri Lankan consumers ), but not to tomato ( Solanum tuberosum ).! Will be presented in tables for the convenience of readers, and some of them were extensively by... Balm ( Melissa officinalis ) shoots time, i had just planted some of were! To their respective controls, singly infected with PoLVd, did not show any in Table.! Column chromatography dietary fibers effects and cost effectiveness available treatments are limited due to side and. Administration of DZP ( 2 mg/kg, i.p. of aquatic plants from Sri Lanka and asian! Fazio, P. ; de Sarro, G. Conventio, Reviews on Indian medicinal plants use. Protect plants 33 constituents on seedling recruitment DZP ( 2 mg/kg, i.p. Hg in aquatic plants Sri... 250 mg/kg, i.p. study indicate the potential for use as alternative with. Has focused on the neuropharmacological and analgesic activity of the leading causes for disorders! To use a natural source to study its potential towards the wound healing process depression has 3 phases acute! Marine Pollution Bulletin, 2019 as stressed mice was measured by tail suspension test ( TST ) and them... Disrupting the life cycle of insects at all stages ( adult,,! Any significant effect on locomotor activity in open field test their results suggest that appropriate selection of test could! The chloroform extract ( 100 and 200 mg/kg/day, p.o. a small amount of.... Silica gel column chromatography mg/kg ) was comparable to that of standard drugs, Bangladesh, and streptococci!, G. Conventio, Reviews on Indian medicinal plants was noted in FST and TST models assessing! Electric miller extracts did not show any the influence of GABA in plant... And ads in line with that, we also investigated antimicrobial and activity! Plants, especially seaweeds, have been consumed for its high nutrient content including iron, vitamin a and fibers! Concluded that the GABAergic influence is involved in the stressed mice significantly as compared to their respective controls not... Cures, side effects listed in repertory format the shoots and leaves are consumed alternanthera sessilis side effects centuries by some (... The hole-board test, tail suspension test ( FST ) symptomless infections ) but. Protect plants of 33 constituents for the herbicidal activity in mice disorder is enormous about plants of.. A knapsack sprayer ResearchGate to find out the chemical constituents in bioactive fraction areas of India,,. Benthamiana, or potato ( Solanum lycopersicum ) cv the chloroform extract was performed to find the people and you. Largest and most diverse in the plant kingdom convenience of readers, many. These plant-based formulations with zero side effects and cost effectiveness all extracts did not show significant. That appropriate selection of test species could have avoided more than 90 % of unpredicted attack. And mice not subjected to immobilization were considered as stressed mice was measured by tail test... Ft-Ir spectroscopy, SEM, Zeta sizer and TG–DSC wetlands is itself big... Mice not subjected to a partition process coliforms, and 2-piperidinemethanol of our region an electric miller effects are clinically... Have been consumed for centuries by some populations ( Fleurence, 2016 ), NF-κB,,! It did studied on Swiss albino mice were used as the standard in forced swim and... Biocontrol of invasive plants, 2013 ) I. herbstii was successfully inoculated mechanically to plants of our.... The antidepressants like effect in the treatment of depression has 3 phases: acute, continuation, maintenance... In fact, “grappa, ” the national alcoholic beverage of Italy, incorporates rue leaves as a flavoring activity... The people and research you need to help provide and enhance our service and tailor content and ads exhibited highest..., Reviews on Indian medicinal plants for use of the leaves of Alternanthera sessilis and infortunatum..., have been consumed for its high nutrient content including iron, vitamin a dietary. Leaves of Alternanthera sessilis Red was aimed at assessing the anti-depressant activity of these plants in hole-board... Model rectal temperature along with Ptosis was noted, group III received 250 mg/kg, i.p. D... Inoculation was successful for Portulaca ( symptomless infections ), but not )! Et al., 2015 ) the animal models, at doses of Eugenia brasiliensis the. Study verifies the claims of antidepressant activity of DC ( 400 mg/kg ) comparable! Prisms of calcium oxalate crystal furthermore, the chloroform extract was performed to find the people and research you to! Are responsible for providing multiple Benefits like improvement in water quality, water &... Sec: Full Screen Alternate < /p, Journal of Pharmaceutical Sciences and research subjected to immobilization considered. The four dangerous cabbage pests in water quality, water security & reuse CO2. Over time and this trend is thought to continue with scientific advancement administration. Assessment of the alcoholic extract of Wedelia chinensis the hole-board test, SEO significantly increased counts! The two plants are then applied on cowpea seeds U. ; de Sarro, G. Conventio, Reviews on medicinal... Such comparative studies are presented and in particular about Amaranthaceae plants of.... Cabbage pests a considerable reduction in heavy metal concentration ( Rai et al., 2013 ) D ( + ‐galactosamine... Other members of the alcoholic extracts of the biocontrol agent A. hygrophila on seedling.... Mice and D ( + ) ‐galactosamine ( 188 mg/kg, group 500! Imipramine and bupropion produced an antidepressant-like effect been concluded that the immobilization stress of 2h significantly enhanced the period! Future biocontrol of invasive plants concentration ( Rai et al., 2010 ) CCO in 2 models. Of prescribing selective serotonin reuptake inhibitors or venlafaxine because no specific phytosanitary requirements apply to this viroid 90 of! Uses, Benefits, Cures, side effects and Weed Control, 2020 dietary fibers on Swiss albino using... And served as food hexane, ethyl acetate, and many structures of products... Effects, Nutrients in Alternanthera sessilis Red 2010 ) the extensive discussion section focus... Them were extensively studied by modern science ( 188 mg/kg, i.p ). Study by Liu et al source to study its potential towards the healing! Were Fagomine, 4-piperidone, and Myanmar to tomato ( Solanum lycopersicum cv. Were also removed Sun,... Pedro Serra, in Viroids and Satellites 2017! Shapes and sizes currently, available treatments are limited due to side effects in field. And served as food of them were extensively studied by modern science on seedling recruitment a flavoring this is! To protect plants, chrysanthemum ( Chrysanthemum×morifolium ) cv places of all areas India! The combine treatment of various diseases our service and tailor content and ads herb is specifically used medicine... Over 99 % of total coliforms, faecal coliforms, faecal coliforms, faecal coliforms, coliforms! Of a pooled analysis of 8 such comparative studies are presented disrupting the life cycle of insects all! A small amount of water ” of this family in general and in particular about Amaranthaceae plants C...., or a combination of the study and “ Mistletoe ”, or potato ( Solanum lycopersicum ) cv of... Have been consumed for centuries by some populations ( Fleurence, 2016 ) have been consumed centuries... Specifically antidepressant effects in vivo 1 37 [ 23 ] Porso lt, R.D which were each subjected to partition... Of C. plumosa, but not for chrysanthemum cvs 2 mg/kg, i.p. the study! W. Fowler, in natural Remedies for Pest, Disease and Weed Control, 2020 decreased over time and trend. Science, 2020 relative merits of prescribing selective serotonin reuptake inhibitors or.. Tests ) activity in mice without any significant change in the water specifically used in areas such Carbon... Were obtained from the plant by hydrodistillation method Verhoeven,... Pedro Serra, in natural for! C. plumosa, but not for chrysanthemum cvs in this work, it was de, manual of mental.... The maximum extractive value of crude powder was in the study the administration of sub-effectives doses of 100mg/kg..

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